Product Category
Products > Biolab Use EnzymesTrypsin ELISA Kit
Name:Trypsin ELISA Kit
Description: The Kit is for the quantitative determination of trypsin in final product which is produced using trypsin in manufacture process. The assay range is 5~250ng/ml.
 

Description

        The kit uses anti porcine trypsin antibody as the coating antibody, the enzyme labeled anti porcine trypsin antibody as the detection antibody, the highly sensitive TMB as the substrate, and the porcine trypsin as the standard to quantitatively detect porcine trypsin (including recombinant porcine trypsin) in the sample. This kit is characterized by good specificity, high sensitivity and stability,

        The lowest detectable level of porcine trypsin is 0.2ng/ml.

Principle

        This kit uses double antibody sandwich method to detect trypsin residuals. The porcine trypsin antibody is coated on the microplate, and the trypsin in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled trypsin antibody is added for incubation, and the enzyme labeled antibody is combined with trypsin. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The trypsin concentration of the unknown sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.

Kit components

Components

QTY

Package

Anti-trypsin antibody coated microtiter plate

12 strips

96T

Porcine trypsin STD(1000ng/ml)

1 vial

100μl/vial

STD dilution buffer

1 bottle

10ml/bottle

HRP-labeled antibody(100X)

1 vial

100μl/vial

HRP-labeled antibody dilution buffer

1 bottle

10ml/bottle

Substrate solution

1 bottle

10ml/bottle

Stop solution

1 bottle

10ml/bottle

Wash buffer concentrate(20X)

2 bottles

25ml/bottle

COA

1 copy

 

Manual

1 copy

 

 

Specificity  

        Control abs (NSB)                       <0.300
        10ng/ml std abs                           >0.700
        Precision,%CV average <9.0%
        Detection limit (>control 3SD) <0.6ng/ml
 
Storage and stability

        Antibody-coated plate, HRP labeled antibody and trypsin standards are stored at -20℃, other components are stored at 2-8℃. It is stable for 6 months.

Catalog #

        TE-19-002