Product Category
Products > Biolab Use EnzymesCarboxypeptidase B ELISA Kit
Name:Carboxypeptidase B ELISA Kit
Description:The Kit is for the quantitative determination of Carboxypeptidase B in final product which is produced using Carboxypeptidase B in manufacture process. The assay range is 5~500ng/ml.
 

Principle of the test
        The Carboxypeptidase B test uses a double-antibody sandwich ELISA to analyze the residual amount of Carboxypeptidase B in samples. First add sample to 96-well plate pre-coated with one Carboxypeptidase B specific antibody to capture available Carboxypeptidase B in solution. Standards and samples are added to the wells, and any Carboxypeptidase B present binds to the immobilized antibody. The wells are washed and HRP conjugated anti-Carboxypeptidase B antibody is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and HRP substrate solution is added. When stopping solution is added, it produces a yellow color. Its  intensity proportionally correlates to the concentration of the Carboxypeptidase B in samples.
Usage
        The Kit is for the quantitative determination of Carboxypeptidase B in final product which is produced using Carboxypeptidase B in manufacture process. The assay range is 5~~500ng/ml.

Materials supplied   

1

 Precoated-plate

8-well X 12 strips

6

 5 X Substrate A

2 mL

2

 CPB Std  ( 0.2mg/mL)

50 μL

7

 Substrate B

500 μL

3

 20 X Wash Solution

20 mL

8

 Stopping Solution

10 mL

4

 Diluent

20 mL

9

I nstruction

1

5

 HRP-conjugated Ab

10 μL

10

 Plate Closure Membrane

1

Example result


Storage

        When received the kit, Carboxypeptidase B standard and HRP-conjugated Ab are placed at -20℃, other compents are stored at 4℃.