Product Category
Products > Biolab Use EnzymesRNase A ELISA kit
Name:RNase A ELISA kit
Description:
 

Description

        The kit uses anti RNase A antibody as the coating antibody, the enzyme labeled anti RNase A antibody as the detection antibody, the highly sensitive TMB as the substrate, and RNase A as the standard to quantitatively detect RNase A in the sample. This kit is characterized by good specificity, high sensitivity and stability,

        The lowest detectable level of RNase A is 0.2ng/ml.

Principle

        This kit uses double antibody sandwich method to detect RNase A residuals. The RNase A antibody is coated on the microplate, and the RNase A in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled RNase A antibody is added for incubation, and the enzyme labeled antibody is combined with RNase A. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The RNase A concentration of the sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.

Kit components

Components

QTY

Package

Anti-RNase A antibody coated microtiter plate

12 strips

96T

RNase A STD

1 vial

100μl/vial

STD dilution buffer

1 bottle

10ml/bottle

HRP-labeled antibody(100X)

1 vial

100μl/vial

HRP-labeled antibody dilution buffer

1 bottle

10ml/bottle

Substrate solution

1 bottle

10ml/bottle

Stop solution

1 bottle

10ml/bottle

Wash buffer concentrate(20X)

2 bottles

25ml/bottle

COA

1 copy

 

Manual

1 copy

 

 

Specificity

 

        Control abs(NSB)                    <0.300
        6ng/ml std abs                             >0.700
        Precision,%CV average <9.0%
        Detection limit (>control 3SD) <0.2ng/ml
 
Storage and stability

 

        Antibody-coated plate, HRP labeled antibody and RNase A standards are stored at -20℃, other components are stored at 2-8℃. It is stable for 6 months.

Catalog #

        TE-19-009