Product Name
        Recombinant human pyruvate kinase M2 from E.coli.
General description
        Pyruvate kinase (PK) is a key rate-limiting enzyme of glycolysis which catalyzes the final step of glycolysis. It converts phosphoenolpyruvate (PEP) to pyruvate while phosphorylating ADP to ATP. There are four isoforms of pyruvate kinase, including PKL, PLR, PKM1 and PKM2. PKM2 is expressed in β-cells, where it is the most abundant glycolytic enzyme. Unlike the other isoforms, the catalytic activity of PKM2 is directly linked to the enzyme's quaternary structure; tetrameric PKM2 has high catalytic activity, whereas monomeric and dimeric PKM2 have low catalytic activity. It is considered as a key regulator of Warburg effect. PKM2 also functions as a transcriptional co-activator or a protein kinase to regulate tumorigenesis. PKM2 is expressed in some differentiated tissues, such as lung, fat, retina, and pancreatic islets, as well as in all cells with a high rate of nucleic synthesis, such as normal proliferating cells and embryonic cells. Regardless of tissue origin, most cancer cells have increased expression of PKM2, which positions PKM2 as an attractive target for cancer therapy.This product is expressed in E.coli and remove His tag during downstream purification process.
Appearance
        Sterile Filtered clear solution.  
Specifications
                 M. W.： Approximately 58 kDa containing 530 amino acids.
                 Quantity： 100μg
                 N-terminal： SKPHSEAGTAFIQTQ
                 Purity： > 95 % by SDS-PAGE and HPLC analyses.
                 Formulation： 0.2μm filtered concentrated solution in PBS, pH 7.4, 20% glycerol.
                 Endotoxin： Less than 1 EU/μg of rHuIFN-γ as determined by LAL method.
Storage
        It is stable at 2-8℃, but should be kept at -20℃ for long term storage, preferably sealed. The diluted preparation is stable for up to one week at 2-8℃. Avoid repeated freeze/thaw cycles.
Usage
          This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.