Description:Optimized ready-to-use PCR mixture of pfu DNA Polymerase, PCR buffer, MgCl2 and dNTPs.
Description
The 2xpfu PCR Master is an optimized ready-to-use PCR mixture of pfu DNA Polymerase, PCR buffer, Mg2+ and dNTPs. The 2xpfu PCR Master contains all components for PCR, except DNA template and primers. Pfu DNA polymerase is thermostable with both 5’- to 3’- DNA polymerase and 3’- to 5’-exonuclease activity. Thus its fidelity of DNA synthesis is much higher than that of Taq DNA polymerase.
Package
2xpfu PCR Master 1mL,40 runs
Conponent
Pfu DNA polymerase(2.5U/25ul);
4 dNTPs(each 0.4mM);
Mg2+(4mM).
Protocol
1、All solutions should be thawed on ice, gently vortexed and briefly centrifuged. Add in a thin walled PCR tube on ice, for a total 50μl reaction volume,each tube 25ul, then store at -20℃,avoiding frozen-thawn repeatedly;
2、For 25ul 2xpfu PCR Master, add forward primer, reverse primer and template DNA, then add water (nuclease-free) to total volume 50ul;
3、Place samples in a thermocycler and start the PCR program;
4、Agarose gel electrophoresis analysis of PCR product.
Storage
Store at -20℃.