Product Category
Products > Biolab Use EnzymesTrypLE Trypsin ELISA kit
Name:TrypLE Trypsin ELISA kit
Description:
 

Description

        The kit uses anti TrypLE trypsin antibody as the coating antibody, the enzyme labeled anti TrypLE trypsin antibody as the detection antibody, the highly sensitive TMB as the substrate, and the TrypLE trypsin as the standard to quantitatively detect TrypLE trypsin in the sample. This kit is characterized by good specificity, high sensitivity and stability,

        The lowest detectable level of TrypLE trypsin is 0.1ng/ml.   

Principle

        This kit uses double antibody sandwich method to detect TrypLE trypsin residuals. The TrypLE trypsin antibody is coated on the microplate, and the TrypLE trypsin in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled TrypLE trypsin antibody is added for incubation, and the enzyme labeled antibody is combined with TrypLE trypsin. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The TrypLE trypsin concentration of the sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.

Kit components

Components

QTY

Package

Anti-TrypLEtrypsin antibody coated microtiter plate

12 strips

96T

TrypLE trypsin STD

5 vials

1ml/vial

TrypLE trypsin reference

1 vials

1ml/vial

Sample dilution buffer

1 bottle

10ml/bottle

HRP-labeled antibody(100X)

1 vial

100μl/vial

HRP-labeled antibody dilution buffer

1 bottle

10ml/bottle

Substrate solution

1 bottle

10ml/bottle

Stop solution

1 bottle

10ml/bottle

Wash buffer concentrate(20X)

2bottle

25ml/bottle

COA

1copy

 

Manual

1 copy

 

 

Specificity

 

         Control abs(NSB)                    <0.300
         3.2ng/ml std abs                           >0.700
         Precision,%CV average <9.0%
         Detection limit (>control 3SD) <0.1ng/ml
 
Storage and stability

 

        Antibody-coated plate, HRP labeled antibody and TrypLE trypsin standards are stored at -20℃, other components are stored at 2-8℃. It is stable for 6 months.

Catalog #

        TE-19-007